In recent years, with the progress of science and technologies and medical research a greater level, from the natural plant extract active ingredient pharmaceutical analysis and develop new drugs has become 1 of the hot. Activity of enzyme induction technologies is a plant biotechnology engineering of new technologies, it from the perspective of active enzyme induction start to promote the bio-synthesis of a huge number of the active ingredient in vivo. Zhejiang University, Biomedical Engineering and Instrument Science, Associate Dean Professor Tian Jingkui said that it will apply the new biotechnology, the field of standard medicine, the activity of enzyme induction technologies, will become the promotion of pharmaceutical manufacturing and plant extracts industry booster.

Extraction of active ingredients face difficulties

In plants there are some quite powerful pharmacological effects of active ingredients, such as the yew roots, stems and leaves contain a kind of taxol can inhibit tumor of tricyclic diterpenoids. Even so, these active ingredients in plants is quite low levels, becoming a bottleneck to limit its extraction and purification. For example, in the Mandia Taxus paclitaxel content of the foliage of only about three ten thousandths, separation and purification very tough.

“Bio-pharmaceutical industry soon after years of investigation, the accumulation and use of some industrial production methods is a comparatively mature, but they are quite cumbersome simply because of its actions, making the high cost of pharmaceutical items.” TIAN Jing-kui said. Bio-pharmaceutical technologies companies, analysis institutes, have never stopped a strong pharmacological effects of these active ingredients extraction tactics, with a view to simplify the extraction method as considerably as probable to decrease production costs.

TIAN Jing-kui, at present the approach in the study focused on extraction technology and method, there are also plants by enhancing the cultivation of medicinal plants, techniques to enhance its active ingredients. For example, in the extraction of artemisinin, by way of taking polyploid induction and related cultivation methods to develop new crop strains of artemisinin. The technologies can overcome the artemisinin content of crops under natural conditions is low, the quality troubles of instability and enhance the artemisinin content of crops and shorten the growth cycle, so as to achieve increased levels of raw materials, lower production cost.

Inventive activity of enzyme induction methods

“Compared with the prior studies described, the active enzyme induction technologies is various from the extraction process, as nicely as the transformation method, but also various from the improvement of medicinal plants, plant cultivation techniques of innovation, but rather yet another way, via the activity of enzyme induction in vivo biological activity of a huge number of synthesis ingredients. “TIAN Jing-kui stated in conjunction with their function.

The technology consists of three studies. First, the natural item-depth study of the mechanism of secondary metabolites. Such as via the yew, mulberry leaf, ginkgo leaf, the active ingredient artemisinin biosynthesis of key enzymes in the analysis, to clarify the relevant biological activity of natural products synthesis mechanism. Second, the orientation of biological active substances inducing. Such as through the optimization of induction conditions, to carry out taxol, artemisinin, ginkgo flavonoids, camptothecin-induced activity of enzymes targeted experiments, to increase the yield of active ingredients. 3 is a significant efficacy, the structure induced by novel compounds directed. Activity of enzyme induction in the course of the study, will get some new structure of secondary metabolites. Such as to induce from the ginkgo leaf out of sulfur-containing aromatic compounds, there is a good antioxidant and antibacterial effects from mulberry leaves Induced Sangpi contained only in the Diels-Alder sort adducts in biology, there are anti-oxidation, anti-inflammatory effects.

TIAN Jing-kui, etc. with the relevant units by means of long-term scientific research, by optimizing the activity of enzymes induced by a variety of technical conditions so that a selection of medicinal plants have been a really low content of active ingredients to be a a lot more substantial increase. To Ginkgo biloba, for example, by means of the activity of enzyme induction technology, an boost of flavonoid content. Among them, an boost of 22% quercetin and isorhamnetin increased by 17%, kaempferol increased by 13%. Pilot-scale production of studies have shown that the application of the technologies content of total flavonoids can boost by 30% ~ 60%. This technologies of Ginkgo biloba for drug processing and production gives a very good technological base, greatly enhancing production efficiency, lessen production costs. Once again, through the activity of enzyme induction, Mandia of taxol content in Taxus in a steady enhance in far more than 70% of artemisinin content of Artemisia annua a steady increase in much more than 100%.

Industrial development of promising

TIAN Jing-kui stated that as a new biological engineering technology, active enzyme induction as compared with traditional methods have more benefits, for the comprehensive development of medicinal plants and utilization of excellent significance for the development of the industrialization of medicinal plants has a quite essential facilitating role.

The technology features and benefits of the maximum extraction rate. Of raw supplies, such as Taxol yew trees, is the world’s rare and endangered species, in our country as his property a protected plant. Although there had been four kinds in our country, and a selection of yew, but the overall view [FS: PAGE], the plant’s resources are extremely scarce, and slow growth. Coupled with the bark of Taxus paclitaxel were very low (about .02%), complex chemical synthesis steps, every 10 tons of bark in order to extract 1 kg of paclitaxel, resulting in shortage of the drug is very expensive. By means of active enzyme induction technology, the bark of the yew taxol content increased 70%, so that it can significantly reduce the exploitation and utilization of tree species.

For Artemisia annua and artemisinin, the conventional technique of cultivation is primarily via different measures to enhance the production of Artemisia annua and artemisinin content. Nevertheless, the above method due to temperature, light and other conditions, coupled with the promotion of medicinal plant cultivation tactics demand extensive technical training, etc., the price is large and the result is not satisfactory. The Extraction of artemisinin-induced enzyme activity does not demand a lot of technologies promotion, does not demand input of plant cultivation, only a technical induced by pharmaceutical firms to total.

Ginkgo biloba extract flavonoids from the traditional approach is referred to by alcohol macroporous resin purification, though to be powerful in extract of Ginkgo biloba in the pharmaceutical active ingredients flavonoids and terpene lactones, flavonoid content, but still reasonably low, only at 24% or so. The activity of enzyme induction technologies can be utilized for the Ginkgo biloba extract flavonoid compounds offer a new way.

In addition, the simple method, low energy consumption is also the activity of enzyme induction technologies benefits. As the activity of enzyme induction technologies is only for enzyme induction activity of medicinal plants, and will not result in extraction of the complex and processes on the complex, pharmaceutical organizations can only invest a modest quantity of equipment, technical method is quite simple.

Nonetheless, TIAN Jing-kui said, this technology there are some shortcomings, such as only the roots, stems, branches, leaves, skin kind medicines very good effect, while for fruit, flowers and seeds, the effects in general medicine. At present, they are also becoming increased study efforts with a view to enhance the good quality of these medicines on the breakthrough.